Nadal Lab

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    • Chemical Biology
    • Chemical Proteomics
    • Multiple Myeloma
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      • Simon Nadal
      • Karine Guitot
      • Damia Lounas
      • Maki
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      • BioCIS - Cergy
      • BioCIS - Saclay
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Research environment

Equipment, infrastructures and expertise supporting our interdisciplinary research.

We are an interdisciplinary research group in chemical biology and as such require access to a wide range of equipments and support facilities spanning several 'traditional' disciplines. The following section details the equipment we have access to and what's possible in our lab.

Hosting at CYU / BioCIS

Our research group is located at CY Cergy Paris University on the science campus of Neuville-sur-Oise, 35 min. West of Paris by train. We are part of the larger BioCIS mixed research unit (UMR 8076: Biomolecules: Design, Isolation, Synthesis) which focusses on synthesis of biologically relevant molecules, natural product chemistry, medicinal chemistry and chemical biology. Our research group is affiliated to the Department of Chemistry at CY Cergy Paris Université, the Faculty of Pharmacy at the University of Paris-Saclay (France's largest research university), and the Institute of Chemistry of the French National Center for Scientific Research (CNRS).

Organic chemistry

BioCIS members have access to state-of-the-art facilities for synthetic organic chemistry. This includes synthetic equipment such as synthetic fumehoods, evaporators, Schlenk lines, microwave reactor, automated synthetisers, photoreactors, electrochemistry kits, hydrogenation station, lyophilizer, glovebox, as well as purification equipment including MPLC flash chromatography (x2), analytical HPLC, semi-preparative HPLC, preparative HPLC equipped with UV and ELSD detectors and chiral HPLC columns. All required analytical equipment and services are available for compound characterization at BioCIS including NMR spectrometers (200-400 MHz), mass spectrometry (GC-MS, LC-MS, HPLC-MS, high resolution MS, MS/MS), infrared spectrometry, UV-VIS spectrometers, polarimeters, melting point instruments, coulometers, atomic adsorption spectrometers and an elemental analysis service.

  • Equipment BioCIS-CY
  • Equipment CYU Chemistry
  • HRMS analysis
  • Elemental analysis
  • Glass Blower

Peptide synthesis

BioCIS has access to state-of-the-art facilities for in-solution peptide chemistry as well as access to the PeptLab platform for solid-phase peptide synthesis (SPPS). This platform is fully equipped for milligram and gram-scale peptide synthesis (SPPS), including all equipment necessary for purification (preparative HPLC, semi-preparative HPLC, lyophiliser) and analytics (LC-MS). The PeptLab platform equally possesses a kilo-lab for peptide synthesis in a clean environment. The platform head (Elisa Peroni) and research engineer (Olivier Monasson) provide expert advice in peptide synthesis. Furthermore, BioCIS-CY hosts several research groups with expertise in the synthesis of unnatural amino acids, particularly of fluorinated amino acids, and the coupling reactions necessary to incorporate these (sometimes challenging) building blocks.

  • SPPS synthetisers

Biochemistry & protein chemistry

The 40 m2 protein chemistry laboratory possesses all required equipment for recombinant protein expression in E. Coli (registered L1-1102). We have access to 2 small autoclaves and 1 large autoclave in the service rooms of the Biology Teaching Labs (SPEX) for sterile media preparation and sterila consumables. Our lab is well-equipped for molecular biology and protein purification, including thermal cyclers for PCR, thermoshakers, bacterial incubators, sonicator, centrifuges, electrophoresis, an AKTA Start FPLC purification system with SEC, SCX and affinity chromatography columns, -20 and -80 freezers.

One of the specificities of our lab is the implementation of post-translational chemical mutagenesis, an intact protein modification chemistry consisting of a series of bioorthogonal reactions. Some reactions require anaerobic conditions (6ppm), which we perform in an acryl glovebox (see picture). We have also built in-house a biocompatible photoreactor for photoredox reactions on proteins.

BioCIS-Cergy possesses a Tecan plate reader for enzymatic assays (ELISA), a spectrofluorometer with plate reader, an ITC for protein-ligand interaction and a Q-TOF tandem mass spectrometer for intact protein LC-MS.

  • Biochemistry Equipment
  • Lab tour (fr)

Chemical Biology

As described, we have access to a wide range of state-of-the-art facilities for chemical biology.

The BioCIS compound library is part of the 'Chimiothèque Nationale', the French National Compound Collection. Compound collections in plates are available at request (standardised subsets or cherry-picked) from ChemBioFrance or from the BioCIS lab collection. BioCIS is a founding member of the CIBLOT high-throughput screening (HTS) platform where assays amenable to HTS using absorbance, luminescence and fluorescence can be implemented (colorimetric, alphascreen, FRET/BRET).

Computational chemistry ressources (including molecular dynamics, docking etc.) can be accessed at the local computing center (CDC), which is well-maintained and has installed all necessary software packages for CPU and GPU computing. While the local computing is entirely sufficient for our needs, we qualify for the free access to the French National HPC infrastructure GENCI (Accès préparatoire).

  • Chimiothèque Nationale
  • HTS equipment (CIBLOT)

Cell biology

2D mammalian cell culture is carried out in a BSL-2 biosaftety lab at Neuville sur Oise. We have access to a dedicated BSL-2 lab module of the Cosmetomics platform under a pay-for-access scheme. The lab is fully equipped for mammalian cell culture laboratories (including laminar flow hoods, incubators, centrifuges, autoclave etc.) and is conveniently located in the same building complex as BioCIS. The platform manager as well as a PhD-level research engineer provide technical support. BioCIS also shares a cell culture platform in Saclay with the Institut Gallien, with a full-time research engineer position providing support.

The ERRMECe research group and the CY Advanced Microscopy platform are also located on campus, providing further expertise, support staff and access to state-of-the-art microscopy equipment.

  • Cell culture in Neuville
  • Cell Culture in Saclay
  • Advanced Microscopy
  • Flow Cytometry

Mass Spectrometry

Due to our interdisciplinary research, we have a broad need in different mass spectrometry techniques. We have direct access to the following instrumentation:

  • GC-MS of the Departement of Chemistry (AGILENT HP6890/5973N)
  • UPLC-MS of PeptLab (Waters 3100)
  • UPLC-MS/MS Q-ToF (Waters) of BioCIS
  • Pasteur Proteomics Facility

Hosting partners


The BioCIS UMR

Large research unit (118 members) affiliated to CNRS, Paris-Saclay and CYU (ID: UMR 8076). Medicinal chemistry and synthetic methodology for bioactive NCEs.

  • BioCIS website

BioCIS Team 5: Biological Chemistry

Located at CYU, focus on amino acid, peptide, protein and carbohydrate chemistry. Structured in 6 research groups; we are the 'protein chemistry' group.

  • BioCIS-CY website

PeptLab: Peptide Synthesis

Small Research Facility (SRF) / platform providing services in peptide synthesis. Strongly interlinked with BioCIS-CY.

  • Peptlab website

Cosmetomics: cell culture

SRF / platform providing services in cosmetics. We have access to the BSL-2 lab for cell culture.

  • Cosmetomics

Support Facilities


IPSIT platforms

BioCIS is a founding members of IPSIT, a federation consisting of several SRFs for preclinical drug discovery. As members of BioCIS, we have a preferential access to its services.

  • IPSIT

HTS Screening: CIBLOT

HTS Screening platform located at Paris-Saclay.

  • CIBLOT

Biophysics: INTERMOL

While we have an ITC in our lab, the INTERMOL platform provides access to a Biacore T100 for SPR.
We can also access the molecular biophysics platform at I2BC at preferential rates through IPSIT.

  • SPR - INTERMOL
  • I2BC Biophysics

The research group

We develop chemical biology and chemical proteomics methods for biomedical research, with an emphasis on multiple myeloma. We are part of the larger BioCIS mixed research unit (UMR 8076 - synthetic biomolecules), affiliated to the CNRS, the University of Paris-Saclay and CY Cergy Paris University.

  • BioCIS - Institute website
  • BioCIS - Chemical Biology Team
  • Address

    CY Cergy Paris Université
    Laboratoire BioCIS
    5 mail Gay-Lussac
    Neuville sur Oise
    95000 Cergy-Pontoise
    FRANCE

  • Phone

    Office (F2.37):
    +33 (0)1 34 25 70 49
    Lab (F2.24):
    +33 (0)1 34 25 70 53

  • Email

    simon.nadal [at] cyu.fr

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