Nadal Lab

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      • Karine Guitot
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    Please note: This site is under construction.

Chemical proteomics

Exploring molecular interactions in the proteome using novel chemical tools.

Chemical proteomics focuses on creating chemical tools and chemical probes tailored for the use in proteomics assays.

Protein-ABPP: Protein-based activity profiling using reactive covalent protein probes

The recent discovery of methods for the incorporation of reactive amino acids into proteins through posttranslational chemical mutagenesis[1] has opened new possibilities for the study of protein-protein interactions and the investigation of molecular interactions. For this, reactive mimics of amino acids are incorporated in vitro using synthetic protein chemistry methods into target proteins. These mimics substitute either a canonical amino acid or an amino acid with a posttranslational modification such as acetylation, methylation, phosphorylation or glycosylation. Once incorporated, protein probes with reactive amino acid mimics can be used to covalently trap protein-protein interaction partners via proximity-enabled protein-protein crosslinking (PEPC). The reaction between the probe and its interaction partner is specific for the site of interaction (the binding site). Combined with a crosslinking mass spectrometry readout, these reactive protein tools allow to probe molecular interactions with amino-acid-level precision.

We develop tailored chemical proteomics methods in crosslinking mass spectrometry to be able to identify site-specific crosslinking events between reactive proteins and their interaction partners. We work on all aspects from sample preparation, data acquisition by high resolution mass spectrometry and data analysis.

Affinity matrices for the enrichment of subproteomes

We are starting projects developing affinity matrices to enrich for specific posttranslational modifications and proteomes, including:

  • Protein O-glycosylation.
  • Interaction partners for immunogenic antigens.

References

  1. Josephson B, Fehl C, Isenegger P, Nadal S, et al. “Light-driven post-translational installation of reactive protein side chains.” Nature 2020, 585, 530.

Proteomics at Pasteur

We collaborate with Julia Chamot-Rooke, Martial Rey and the Institut Pasteur proteomics facility for access to mass spectrometry-based proteomics instrumentation. Sample preparation and data analysis is done in our lab at CYU.

  • Our Proteomics Partner

Our datasets

A link to our proteomics data uploaded into PRIDE. (coming soon)

  • Learn More

The research group

We develop chemical biology and chemical proteomics methods for biomedical research, with an emphasis on multiple myeloma. We are part of the larger BioCIS mixed research unit (UMR 8076 - synthetic biomolecules), affiliated to the CNRS, the University of Paris-Saclay and CY Cergy Paris University.

  • BioCIS - Institute website
  • BioCIS - Chemical Biology Team
  • Address

    CY Cergy Paris Université
    Laboratoire BioCIS
    5 mail Gay-Lussac
    Neuville sur Oise
    95000 Cergy-Pontoise
    FRANCE

  • Phone

    Office (F2.37):
    +33 (0)1 34 25 70 49
    Lab (F2.24):
    +33 (0)1 34 25 70 53

  • Email

    simon.nadal [at] cyu.fr

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